The CU Boulder Stem Cell AV名湿 and Technology Resource Center offers CU AV名湿ers and Industries the following services:

Biosafety Cabinets (24/7)

  • Biosafety Cabinet with lab supplies and some consumables
  • Biosafety Cabinet without lab supplies and consumables

*It is allowed to work with any human cell line except human Embryonic Fibroblast or human Embryonic Stem Cells.

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    iPSC lines

    • Thaw iPSCs听
    • Expand and Freeze iPSCs
    • Mycoplasma Testing
    • Surface Stem Cell Markers听
    • Trilineage Differentiation Assay

    iPSC-Derived NPC(neural) and Neuronal Stem Cell听lines

    • Thawing Assay
    • Expansion & Freezing
    • Mycoplasma Testing
    • Surface Stem Cell Markers Assay

    Miscellaneous

    • Cell Proliferation Assay
    • Media changes听
    • Live culture of iPSC lines
    • Fresh Matrigel/Vitronectin/Geltrex pre-coated plates听
    • Generating iPSC cell pellet for RNA/DNA extraction
    • Monthly Core usage charge

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    iPS Cell Line Request

    The Core has several established human Fluorescently and Fluorescently Tagged Human Induced Pluripotent Cell Lines听for purchase for research purposes.听Please contact the director for available cell lines.

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    Other Cell Services

    • 24/7 Remote Cell Culture Monitoring (Olympus Provi CM20)
    • Co2 Shaker with remote controller-Organoid's derivation
    • Nucleofector 2b Device 鈥� Transfection system
    • Evos M7000 Training
    • Olympus IX83 Microscope Training
    • Olympus CKX53 Microscope Training
    • Olympus Provi CM20 Training
    • Neon 听NxT Transfection System Training
    • Amaxa Nucleofector System Training
    • Consulting

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    Incubators

    • Monthly rent
    • Normoxic and Hypoxic Conditions
    • Organoids Derivation
    • Automatic Cell Tracking

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    Fluorescence Microscopes (24/7)

    • Inverted Fluorescence Scanner Microscope (Olympus IX83) with live-cell time-lapse capabilities (Live and fixed cell imaging in monolayer or suspensions, organoids, embryos, organotypic slides, etc.)
    • Sterile dissection hood equipped with a dissecting Olympus CKX53 microscope and heated/CO2听stage for work with live cultures and live/fixed images.
    • Evos 7000 Microscope听with live-cell time-lapse capabilities (Live and fixed cell imaging in monolayer or suspensions, organoids, embryos, organotypic slides, etc.) (1.2X, 2X, 4X, 20X, 40X, 60X, 100X)

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    Biorepository

    • Vial or box for 100 Cryoviales (Price per month or year)

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    Reagents from ThermoFisher听(up to 15听% discount. The final cost already includes shipping costs)

    • Media
    • Kits
    • Supplements
    • Serum
    • Matrices
    • Cytokines
    • Small molecules
    • Free samples
    • Free demos

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    Reagents from STEMCELL Technologies听听(up to 15听% discount. The final cost already includes shipping costs)

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    Kits and services through Synthego Company (up to 10% discount on products and some services).

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    Synthego-Synthetic sgRNA Kits:听

    • High-efficiency guide RNAs for knockout and knock-in edits
      • , design top-scoring guide RNAs for gene knockout
      • , a free online tool that provides an easy knockout score
    • Gene Knockout Kits v2
      • Using our multi-guide design approach for protein-coding genes in human and mouse genome
    • Arrayed CRISPR Screening Libraries听
      • Available in custom, pre-designed, and whole human/mouse genome gene sets

    Synthego-鈥�Standard Edits:

    • Human iPSCs, ESCs, and Immortalized Cell Lines听
      • Fragment deletion听
      • Knock-ins up to 2,000 bp (Tag / Region Insertion)听
      • Single nucleotide variant changes (SNV)
      • Single, Double, and Triple Gene Knock-out (KO)

    Look听听to see Synthego's industry-leading guarantees for our cell-editing services.

    Type of Edit

    Description

    Deliverable Type听

    Turn Around Time

    Gene KO听

    Creating a gene KO using a synthetic guide to make a functional protein knockout. Single, double, triple gene KOs are supported.

    Pool

    8 鈥� 10 weeks

    Clone

    16-20 weeks

    SNV Change

    Changing a single nucleotide variant (SNV) or single nucleotide polymorphism. It can be either a nucleotide or amino acid change.

    Pool

    10 鈥� 12 weeks

    Clone

    16-20 weeks

    Small Tag/ Insertion

    Insert a small tag (100 bp or less) into a genome region.听

    Pool

    10 鈥� 12 weeks

    Clone

    16-20 weeks

    Large Tag / Region Insertion

    Insert a large tag or region of interest (2,000 bp or less) into a targeted genome region.听

    Pool

    16-20 weeks

    Clone

    20-28 weeks

    Synthego-Standard Deliverables for Engineered Cell Projects:听

    鈼徧�2 independent biological clones or 2 cell pools (2 vials of each: 0.5M cells per vial)

    鈼徧�2 vials of wild-type isogenic cells mock-transfected pools, passage matched (0.5M cells per vial)

    鈼徧鼼enotypic Analysis and Editing Summary Report听

    鈼徧鼵ertificate of Analysis

    Synthego-Additional Information:

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    • Learn more about our听听and听
    • Target Discovery with听
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    For pricing information or a quote for a specific project(s), please email听Lucia.GayossoMiranda@colorado.edu听with the following details:听Cell Line,听Knock-out, or Knock-iN. If听Knock-out, please specify the gene name; if听Knock-in, 听type of edit: (i.e., SNV, Myc tag, GFP, etc.). Note: times and dates you are available for a discussion

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    Professional Development

    • hiPSC training: Over 36听hours of training course.听

    It is focused on working with hiPSC in basic and translational research. Lecture topics and hands-on training include iPSC generation and characterization, growth, maintenance, cryopreservation, thawing of hiPSCs, and generation of iPSC-derived cell lines and organoids.

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    Custom Services

    Please contact the Core director if you require custom services not listed above. We will do our best to accommodate you!